不同类型牛GPR54基因启动子变异与其表达水平的关联性

doi:10.11843/j.issn.0366-6964.2016.09.010

Abstract

Abstract:

The aim of the study is to investigate the impact of the variations of GPR54 promoter on the expression levels of GPR54 gene in Holstein (HT)，Wandong cattle (WD) and Jianghuai buffalo (JH)，therefore further unveil the association between the polymorphisms of GPR54 gene promoter and the sexual precocity.Total RNAs in leukocytes of HT，WD and JH were extracted and the polymorphism of the promoter region were detected by PCR and sequencing.Furthermore，the efficiency of the promoters were determined via RT-PCR and luciferase expression from the fusion reporter gene.Various SNPs were detected in the promoter regions of GPR54 genes in HT，WD and JH with significant difference.Moreover，it was shown that the promoter efficiency of HT was 4.44 times and 1.99 times higher compared to JH buffalo and WD respectively.Meanwhile the promoter efficiency of WD was 2.24 times higher than JH.Notably，the RT-PCR results showed that GPR54 gene expression levels in youngsters were dramatically higher than that of the adults especially in HT than in WD and JH.JH showed the weakest expression level of GPR54 while the cows exhibited the strongest GPR54 expression level compared to ox (P<0.01).These results indicated that the polymorphisms of GPR54 promoter and the bovine sexual precocity were evidently correlated.

CLC Number:

S823.2

WANG Xue-gu,DENG Dan-mei，CHENG Jin，WANG Yang，LI Tong，CHEN Hong-quan. The Relationship between the Expression Variations of GPR54 Gene and the Polymorphisms of GPR54 Promoters in Different Types of Bovines[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1824-1829.

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The Relationship between the Expression Variations of GPR54 Gene and the Polymorphisms of GPR54 Promoters in Different Types of Bovines

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